Fig. 8

Histopathological analysis of the presence of eosinophils in uninfected mice. The evaluation was performed by using Sirius Red staining of quadriceps muscles from the back of the hind thigh collected from mice submitted to AWB and SAL therapy. (a-f) Light microscopy analysis and (g) determination of the percentage of eosinophils (mean ± SD) stained by the pink labeling (arrows, a-f) of tissue samples collected from mice submitted to one (a-e) or three (f) cycles of AWB (a, b, f and g) and SAL (c, d and g) injection (20 μL) and monitored at 2 (a, c and g), 24 (b, d and g) and 48 h* (e, f and g) after therapy. Original magnification × 1000 for all panels. Bars represent 20 μm