Table 1 Nucleotide sequences of primers used for RT-PCR and cloning experiments
Primer identification | Nucleotide sequence of primer | Nucleotide positiona |
|---|---|---|
Subunit α | ||
αT-f | 5’-ATGTCTTCAGATTTGGTAATGCCT-3’ | 60–83 |
Catα-f | 5’-CGCAGAGAGAAACTGATCCCA-3’ | 126–146 |
Catαf-r | 5’-TGGGATCAGTTTCTCTCTGCG-3’ | 126–146 |
Deg2-f | 5’-GGGGCMAATGCYTTCTTTGT-3’ | 498–517 |
Catα-r | 5’-ATTGGCTCTCCTCTTCAGTTT-3’ | 900–921 |
Degr-f | 5’-ATTACTGGGAGGTGGAGTGG-3’ | 1790-1809 |
Deg-r | 5’-CCACTCYAMCTCCCAGTAAT-3’ | 1790-1809 |
Synα-r | 5’-TYAAAGTAATCTGASAGTTCC-3’ | 2151-2171 |
Subunit β | ||
βTotal-f | 5’-GTTGGAGTCATGCCTTCAGAC-3’ | 52–71 |
CDβ-r | 5’-GAGCTCACAGTCATACCA-3’ | 1621-1638 |
CDβr-f | 5’-TGGTATGACTGTGAGCTC-3’ | 1621-1638 |
Synβ-r | 5’-TAGTTTAATTTGACCATT-3’ | 2139-2157 |