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Table 1 Nucleotide sequences of primers used for RT-PCR and cloning experiments

From: Sequence analysis of the cDNA encoding for SpCTx: a lethal factor from scorpionfish venom (Scorpaena plumieri)

Primer identification Nucleotide sequence of primer Nucleotide positiona
Subunit α
 αT-f 5’-ATGTCTTCAGATTTGGTAATGCCT-3’ 60–83
 Catα-f 5’-CGCAGAGAGAAACTGATCCCA-3’ 126–146
 Catαf-r 5’-TGGGATCAGTTTCTCTCTGCG-3’ 126–146
 Deg2-f 5’-GGGGCMAATGCYTTCTTTGT-3’ 498–517
 Catα-r 5’-ATTGGCTCTCCTCTTCAGTTT-3’ 900–921
 Degr-f 5’-ATTACTGGGAGGTGGAGTGG-3’ 1790-1809
 Deg-r 5’-CCACTCYAMCTCCCAGTAAT-3’ 1790-1809
 Synα-r 5’-TYAAAGTAATCTGASAGTTCC-3’ 2151-2171
Subunit β
 βTotal-f 5’-GTTGGAGTCATGCCTTCAGAC-3’ 52–71
 CDβ-r 5’-GAGCTCACAGTCATACCA-3’ 1621-1638
 CDβr-f 5’-TGGTATGACTGTGAGCTC-3’ 1621-1638
 Synβ-r 5’-TAGTTTAATTTGACCATT-3’ 2139-2157
  1. aNumbering is based on nucleotide sequence from stonustoxin (Synanceia horrida), accession numbers GenBank: U36237 and U32516 [18]