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Fig. 3 | Journal of Venomous Animals and Toxins including Tropical Diseases

Fig. 3

From: Identification, expression and characterization of the recombinant Sol g 4.1 protein from the venom of the tropical fire ant Solenopsis geminata

Fig. 3

Determination of the overexpression of rSol g 4.1 protein by SDS-PAGE and Western blotting. a Protein expression patterns in BL21(DE3) pLysS competent cells cultured under optimal conditions obtained using SDS-PAGE. Lanes: M – molecular weight standards; 1 – expression without IPTG; 2 – culture grown in the presence of 0.4 mM IPTG for 8 h.; 3 – cell extract in solution; 4 – cell extract in pellet. b Western blot of the rSol g 4.1 protein using an anti-His tag antibody; lane 1 – cells lacking the rSol g 4.1 protein and lane 2 – expression of the rSol g 4.1 protein

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