Fig. 6

Allergenic analysis of native and recombinant Sol g 4.1 with anti-Sol g 4.1 IgE antibody. a Crude venom expression pattern, as determined by SDS-PAGE. b Determination of the allergenic properties of the Sol g 4.1 protein by producing an antiserum in mice and analyzing the product using Western blotting. Recognition of native Sol g 4.1 and rSol g 4.1 proteins by serum IgE in Sol g 4.1 protein-sensitized mice. Serum samples: P1-P3 = individual sera of Sol g 4.1 protein-sensitized mice; N1-N3 = serum from mice injected with PBS, acrylamide gel and adjuvant, respectively, as controls