Table 1 List of primers used in PCR and RACE-PCR

Degenerate

 F2 5΄-AAWGTATRAAWACAVHAYC-3΄

 R3 5΄-CKTYTBYCAWTKATTRGTSCC-3΄

RACE

 3RACE 5΄-CGCAGCTGATATTAAGG-3΄

 5RACE 5΄-GTCAATTCGAGCACACCC-3΄

PCR

 FSol4_Nco 5΄-CCATGGCTGCTGATATTAAGGA-3΄

 RSol4_Xho 5΄-CTCGAGTCATTTTTTTTTGCCATAC-3΄

Common

 Oligo (dT₁₈) 5΄-GGCCACGCGTCGACTAGTACTTTTTTTTTTTTTTTTT-3΄

 AAP 5΄-GGC CAC GCG TCG ACT AGT ACG GGI IGG GII GGG IIG-3΄