Fig. 1

(a) PAGE in the presence of SDS and β-mercaptoethanol. Lanes: 1 – standard, molecular-weight markers; 2 – C. gigantea (body-wall); 3 – C. gigantea (total); 4 – M. alcicornes; 5 – S. helianthus; 6 – P. homomalla; 7 – B. annulata; samples were applied containing 500 μg of each extract. (b) Fibrinogenolytic activity of cnidarian extracts.1 – Fibrinogen; 2 – fibrinogen + C. gigantean (body-wall); 3 – fibrinogen + C. gigantea (total); 4 – fibrinogen + M. alcicornes; 5 – fibrinogen + S. helianthus; 6 – fibrinogen + P. homomalla; 7 – fibrinogen + B. annulata. Fibrinogen hydrolysis was demonstrated by SDS-PAGE using 12% polyacrylamide gels