Fig. 8

Light micrographs of mouse diaphragm muscles submitted to hematoxylin and eosin staining. Control muscle (a) and muscles exposed to extracts of C. gigantea (body-wall) (b), C. gigantea (total) (c) and S. helianthus (d). Note the general normal appearance of fibers with polygonal aspect (f) and endomysium (en). Muscle damage index of (C) and (D) (4.3 ± 1.3, n = 5 and 5.5 ± 1.1, n = 4, respectively) were not significantly different from that of (A) (2.0 ± 0.4, n = 5). However, muscle damage of B (5.9 ± 0.8, n = 5) was significantly higher than that of A. (ANOVA, p ≤ 0.05). The remaining cnidarian extracts did not show such activity