Fig. 3

Alignment and primary structure of disintegrins. a Sequence alignment between C. d. collilineatus (C0L2T8) and C. d. durissus (Q2QA03) disintegrins, peptides determined by mass spectrometry analysis (Fr6_MS/MS) and sequence obtained by Edman degradation (Fr6_Nterm) of Fraction 6. b Comparison between the classical RGD disintegrin structure pattern and the determined primary structure of Fraction 6 (LVN disintegrin). Bold – cysteines ©; Black box – consensus of all data sequence; Gray box – consensus of three data sequence; Red – pattern signature of disintegrin domain involving five conserved cysteines forming disulfide bonds; Green - RGD motif site; Blue – disulfide bond pattern of disintegrin structure; x – any amino acid residue