Fig. 4

Functional assays with MDA-MB-231 cells. a Cell viability (%) after 24 h of treatment with disintegrin (0.75–6.00 μg/mL). C (−) – negative control (PBS); C (+) – positive control (MMS – 300 μM). The results were analyzed by ANOVA, followed by Dunett’s post-hoc test (*p < 0.05). b and c Cell migration after different times (0, 4, 8 and 24 h) of treatment with non-RGD disintegrin (3 μg/mL). C (−) – negative control (PBS). The dashed yellow lines delimit the region where no cell growth is observed. Data (n = 3) are presented as mean ± SD, which were analyzed by Student’s t test (*p < 0.05)