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Table 1 Protein recovery (%) during the purification procedure of disintegrin from Crotalus durissus collilineatus venom

From: Cell migration inhibition activity of a non-RGD disintegrin from Crotalus durissus collilineatus venom

Sample

Purification steps

aProtein recovery (%)

Cdc venom

Venom solubilization

100.0

Fraction 2

RP-FPLC on a C-18 column (1st chromatography)

0.94

Fraction 6

RP-FPLC on a C-18 column (2nd chromatography)

0.43

  1. aThe recovery percentage of each peak was calculated by the software Unicorn 5.2 (GE Healthcare) using the relation between the area under the curve of absorbance at 214 nm of the corresponding peak and the sum of the areas of all eluted peaks