Table 1 Tryptic peptides identified through MS/MS analysis
Scan time | Fragmentation mode | MS/MS derived sequence | z | Observed m/z | Calculated m/z | Mass deviation (ppm) | Score |
|---|---|---|---|---|---|---|---|
19.56 | HCD | TFGEWRER | 2 | 540.7645 | 540.7647 | −0.37 | 623.7 |
5.86 | HCD | VLLNR | 2 | 307.7031 | 307.7028 | 0.97 | 309.1 |
28.43 | HCD | SNQDLINVQSAAADTLK | 2 | 894.4609 | 894.4603 | −2.35 | 415.4 |
35.27 | HCD | DYYEMFLTK | 2 | 605.2787 | 605.2785 | 0.33 | 288.7 |
14.12 | HCD | YNGNLNTIR | 2 | 532.7779 | 532.7778 | 0.19 | 476.3 |
10.00 | HCD | NSVGIVQDHSPK | 2 | 640.8334 | 640.8333 | 0.16 | 716.8 |
28.43 | HCD | TRVHEIVNTLNGFYR | 2 | 909.9846 | 909.9841 | 0.55 | 730.8 |
13.42 | HCD | KPQCILNKP | 2 | 549.3105 | 549.3104 | 0.19 | 431.7 |
36.74 | ETD | YIELVVVADHGMFTK | 3 | 574.6362 | 574.6359 | 0.52 | 722.1 |
28.49 | ETD | VHEIVNTLNGFYR | 3 | 521.2755 | 521.2756 | −0.19 | 926.1 |
3253 | ETD | ISHDNAQLLTAIDLADNTIGIAYTGGMCYPK | 5 | 671.3300 | 671.3296 | 0.60 | 1359.6 |
9.63 | ETD | NSVGIVQDHSPK | 3 | 427.5580 | 427.5580 | 0 | 707.5 |
37.16 | ETD | HDENHCHCSASFCIMPPSISEGPSYEFSDCSK | 5 | 755.3034 | 755.3031 | 0.40 | 1248.5 |
32.92 | ETD | TLLIAVTMAHELGHNLGMK | 4 | 517.0287 | 517.0288 | −0.19 | 1093.1 |
9.72 | ETD | RKPQCILNKP | 3 | 418.5764 | 418.5764 | 0 | 545.7 |
19.86 | ETD | KPQCILNKPX | 3 | 404.2375 | 404.2374 | 0.25 | 550.7 |
18.00 | ETD | RKPQCILNKPX | 3 | 456.2714 | 456.2711 | 0.66 | 383.5 |