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Fig. 4 | Journal of Venomous Animals and Toxins including Tropical Diseases

Fig. 4

From: Kn-Ba: a novel serine protease isolated from Bitis arietans snake venom with fibrinogenolytic and kinin-releasing activities

Fig. 4

Kn-Ba neutralization by specific antivenom produced by the Butantan Institute. a The inhibition of Kn-Ba proteolytic activity, pretreated or not with α-Botulinic serum and α-Bitis-arietans antivenom, was performed upon Abz-FRSSR-EDDnp FRET substrate. b The percentage of neutralization was carried out and the assays were carried out in quadruplicate. Results were expressed as specific activity (UF/min/μg) ± SEM and analyzed statistically using One-Way ANOVA test followed by Tukey HSD post-hoc tests (*p < 0.05). (*) differences between samples and (#) differences between samples and controls. The hydrolysis of substrate was monitored in the spectrophotometer FLUOstar® Omega (BMG Labtech, HE, Germany; λEM 420 nm and λEX 320 nm); n.s. = not significant

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