Fig. 2

a Chromatographic profile of fraction S3 on a DEAE Sepharose anion exchange column. Elution was initiated with 0.05 AMBIC, pH 7.8, followed by a gradient of AMBIC from 0.05 M to 0.5 M, pH 7.8, and finally 1 M AMBIC, pH 7.8. Fractions of 3 mL/tube were collected at a flow rate of 30 mL/hour. b 12 % SDS-PAGE. Lanes: 1 – tube 15 (D1), 2 – tube 28 (D2), 3 – tube 36 (D3), 4 – tube 40 (D3), 5 – molecular mass standard (260, 140, 100, 70, 50, 40, 35, 25, 15, 10 kDa), 6 – tube 44 (D3-D4 valley), 7 – tube 49 (D4), 8 – tube 55 (D4), 9 – tube 71 (D5a), 10 – tube 77 (D5b)