Fig. 8

The HAase activity. VesT2a: V. tropica venom was tested for specific HAase activity by the (a) zymographic method and (b) optimal pH using turbidimetric assay. For the zymogram HAase activity assay, samples were analysed using 10 % SDS-PAGE containing hyaluronic acid under reducing conditions. The zymgraphic gel was developed overnight at 37 °C under pH 3.7. For the turbidimetric assay, the enzymatic activity of VesT2a, V. tropica venom was tested at various pHs using hyaluronic acid as a substrate at 37 °C