Table 1 Primers and condition of amplification used in the study

DupA 1

CGTGATCAATATGGATGCTT

35 cycles: 45 s, 94°C; 45 s, 52°C and 45 s, 72°C

dupA

Gomes et al.[6]

DupA 2

TCTTTCTAGCTTGAGCGA

Cag1

ATGACTAACGAAACTATTGATC

40 cycles: 1 min, 94°C; 1 min, 53°C and 1 min, 72°C

cagA

Rasmussen et al.[9]

Cag2

CAGGATTTTTGATCGCTTTATT

Hpx1^a

CTGGAGARACTAAGYCCTCC

40 cycles: 1 min, 94°C; 1 min, 59°C and 1 min, 72°C

16S rRNA

Scholte et al. [12]

Hpx2

GAGGAATACTCATTGCGAAGGCGA

SA

ATGGAAATACAACAAACACAC

40 cycles: 45 s, 94°C; 45 s, 54°C and 45 s, 72°C

vacA-s

Atherton et al. [13]

SC^a

CCTGARACCGTTCCTACAGC

Doorn et al. [14]

MA^a

CACAGCCACTTTYAATAACGA

35 cycles at: 45 s, 94°C, 45 s, 55°C and 1 min, 72°C

vacA-m

Doorn et al. [14]

MB

CGTCAAAATAATTCCAAGGG