Figure 4
Stability of purified Hyase after freeze-thaw cycles (-20°C): the activity remained at maximum after the first three freeze-thaw cycles (0, 1 and 3 hours), with a slight decrease after six hours, unchanged until 15 days. Aliquots maintained their activity until the seventh day, losing activity after 15 days of freezing. The points represent mean ± SEM (n = 6), * p < 0.05.