Figure 3

Structure-based sequence alignment of mature truncated and complete hyaluronidases. Full-length hyaluronidases from Echis pyramidum leakeyi (E_pyramidum; Genbank: ABI33941.1), Hy-L-1000 truncated hyaluronidase from Echis carinatus sochureki (E_carinatus; Genbank: ABI33950.1), full-length hyaluronidase from Bitis arietans (B_arietans; Genbank: ABI33945.1), Hy-L-1000 truncated hyaluronidase from Bitis arietans (B_arietans; Genbank: ABI33947.1) and human hyaluronidase (Human_hyal; Genbank: 2PE4). Key catalytic residue is shown in red and positional residues appear in blue. Cysteine residues are marked in black. Secondary structures were based on the human hyaluronidase crystal structure[38]. Secondary structure elements for human hyaluronidase are shown below the sequences: pink arrows represent β-strands, blue cylinders α-helices, and red cylinders 3₁₀ helices.