Bordonein-L, a new L-amino acid oxidase from Crotalus durissus terrificus snake venom: isolation, preliminary characterization and enzyme stability

Table 1 Specific activity and recovery of active fractions eluted during the purification procedure of bordonein-L

Fraction	Total protein (mg)^a	Protein recovery (%)	One unit of LAAO activity (mg)^b	Specific activity (U/mg)^c	Total LAAO activity (U)	Yield (%)	Relative activity
CdtV	556.0	100.0	14.29	0.07	38.9	100.0	1.0
CM5	10.3	1.8	0.29	3.47	35.7	91.8	49.6
CM5S2	4.3	0.8	0.14	6.96	29.9	76.9	99.4
H7 (bordonein-L)	2.7	0.5	0.14	6.96	18.8	48.3	99.4

^aTotal protein quantified by absorbance method 280/205 nm (SCOPES, 1974)
^bLAAO activity unit (U): amount of protein (mg) able to release 1.0 μmol of H₂O₂ per minute
^cSpecific activity: amount of H₂O₂ (μmol) released per minute per mg of protein

ISSN: 1678-9199