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Table 1 Specific activity and recovery of active fractions eluted during the purification procedure of bordonein-L

From: Bordonein-L, a new L-amino acid oxidase from Crotalus durissus terrificus snake venom: isolation, preliminary characterization and enzyme stability

Fraction Total protein (mg)a Protein recovery (%) One unit of LAAO activity (mg)b Specific activity (U/mg)c Total LAAO activity (U) Yield (%) Relative activity
CdtV 556.0 100.0 14.29 0.07 38.9 100.0 1.0
CM5 10.3 1.8 0.29 3.47 35.7 91.8 49.6
CM5S2 4.3 0.8 0.14 6.96 29.9 76.9 99.4
H7 (bordonein-L) 2.7 0.5 0.14 6.96 18.8 48.3 99.4
  1. aTotal protein quantified by absorbance method 280/205 nm (SCOPES, 1974)
  2. bLAAO activity unit (U): amount of protein (mg) able to release 1.0 μmol of H2O2 per minute
  3. cSpecific activity: amount of H2O2 (μmol) released per minute per mg of protein