Fig. 3
a Chromatographic profile of fraction D3 on a C18 reverse phase column. Elution was performed using a RP-HPLC system at a flow rate of 0.5 mL/minute with a linear concentration gradient of 0-100 % solvent B (70 % acetonitrile and 0.1 % TFA) in ten column volumes. b 12 % SDS-PAGE. Lanes: 1 – fraction 1; 2 – fraction 2; 3 – fraction 3; 4 – fraction 4; 5 – fraction 5; 6 – fraction 6; 7 – molecular mass standard (116, 66.4, 45, 35, 25, 18, 4, 14.4 kDa)