Fig. 8

Fibrin(ogen)olytic activity of the MP isolated from B. atrox venom. a Evaluation of the fibrinolytic activity on fibrin gel. Samples were applied on plates containing fibrin gels, and incubation was performed at 37 °C for 24 h. Samples (halo diameter): 1. PBS (0 mm), 2. B. atrox venom 20 μg (15 mm), 3. MP 4 μg (15 mm), 4. MP 6 μg (15.5 mm), 5. MP 8 μg (16 mm), 6. MP 10 μg (16.5 mm). b Evaluation of the fibrinogenolytic activity by SDS-PAGE. Samples were incubated at 37 °C for 1 h and then evaluated on 10 % polyacrylamide gel under denaturing conditions. Lanes: 1 Molecular mass standard (260, 140, 100, 70, 50, 40, 35, 25, 15 kDa), 2. MP 5 μg, 3. Fibrinogen 25 μg, 4. Fibrinogen 25 μg + MP 1 μg