Fig. 1
From: First serine protease inhibitor isolated from Rhinella schneideri poison
RP-FPLC profile and structural characterization of Rs20 by HRESI-MS and GC/MS. a. RP-FPLC on a C2C18 column previously equilibrated with solution A (TFA 0.1 %, V/V). The fractions were eluted with a concentration gradient of solution B (acetonitrile 80 % in TFA 0.1 %, V/V) until it reaches 100 % of solution B (dashed line). Flow rate: 1 mL/minute. b. ESI-microTOF mass spectrum of Rs20. The spectrum indicates the ion masses obtained through positive mode. c. Gas chromatography profile. The spectrum is shown in total ion chromatogram (TIC) × time (minute). d. GC/MS mass spectrum of Rs20. e. GC/MS mass spectrum of lithocholic acid