Fig. 1

Chromatographic profiles of fractions VIIIA and VIIIB from Tsv. (a) Fraction VIIIA. (b) Fraction VIIIB. Fractions (4 mg, eluted of the cation exchange chromatography from Tityus serrulatus venom) were submitted to RP-FPLC on a C18 column (4.6 mm × 250.0 mm, 5 μm particles, Shimadzu Corp.). The column was equilibrated with 0.1 % trifluoroacetic acid (TFA) and the proteins were eluted using a concentration gradient from 0 to 100 % of solution B (80 % acetonitrile (ACN) in 0.1 % TFA), represented by the dashed line. Absorbance was monitored at 214 nm, at 25 °C, using an FPLC Äkta Purifier UPC-10 system. Fractions of 0.3 mL/tube were collected at a flow rate of 0.7 mL/min