Fig. 8
Changes of mitochondrial membrane potential (ΔΨm) induced by F3 fraction in NCI-H358 cells. a Concentration-dependent reduction of red/green fluorescence ratio corresponding to loss of ΔΨm in NCI-H358 cells elicited by increasing concentration of F3 fraction (½ IC50, IC50 and 2 IC50) for 4 h. The results are expressed as the mean ± SD of triplicate determinations, representative of three independent experiments. Significances indicated were in comparison to control cells and to the preincubated cells with NAC for each F3 fraction dose (*p < 0.05; ***p < 0.001 vs. control cells and # p < 0.05; ## p < 0.01; ### p < 0.001, vs. the corresponding dose of F3 fraction with NAC pre-treatment). b Microscopic images of ΔΨm dissipation in NCI-H358 cells after 4 h of treatment with F3 fraction (½ IC50, IC50 and 2 IC50)