Fig. 1

MT-II-induced articular hyperalgesia. MT-II (10 μg/joint) or PBS (vehicle) was injected in tibio-tarsal articulation (25 μL). Pain threshold was determined by a dorsal flexion of the tibio-tarsal joint using a modified electronic pressure-meter test before (time 0 – basal) and 1, 2, 4, 6, 8 e 24 h after MT-II injection, and was represented as force (in g). Zymosan (30 μg) and BSA (20 μg) were used as controls. Each point represents the mean ± SEM of six animals. *p < 0.05 indicate statistically significant differences when compared with PBS group (vehicle)