Fig. 4

Titration of monoclonal antibodies. A 96-well plate was sensitized overnight with one microgram of BthTx-I and venoms from B. jararacussu, southeastern B. jararaca and southern B. jararaca. The plate was then incubated with various concentrations of mAb and revealed with anti-mouse IgG labeled with peroxidase. The arrow in (a) shows the concentration of mAb Bt-3 (0.62 ng/μL) which recognizes BthTx-I and B. jararacussu venom. The venoms from B. jararaca from the south and southeast were not recognized. In (b), titration of mAb Bt-12. Given the reproducibility of the mAbs against BthTx-I, B. jararacussu, southeastern B. jararaca and southern B. jararaca venoms, only a representative result is showed for mAb Bt-3 (representing results from Bt-1, Bt-2, Bt-6 and Bt-10) and for mAb Bt-12 (representing results from Bt-11)