Fig. 2

Bioactivity of purified F11 compounds. a Cation exchange chromatogram illustrating the purification of five compounds from F11 fraction (from 1 to 5 in red). The dashed line illustrates the NaCl gradient used for elution of the compounds. Minor peaks (two before compound 1 and one after compound 5) were not purified. (Inset) Secondary screening of the cation exchange compound 3 (red arrow) on sperm motility parameter VCL. b RP-HPLC purification of cation exchange compound 3. Peaks at elution time 2 min are injection artifacts. c LC-ESI-QTOF MS of compound 3. Inset illustrates the MS of m/z 1048.1010 [7+]