Fig. 3
Chromatographic profiles of horse F(ab’)2 antitoxins and anti-rabies immunoglobulins. (a) Anti-botulinum AB, (b) anti-diphteric, (c) antitetanic and (d) anti-rabies sera were subjected to molecular exclusion chromatography on a Superose 12 HR 10/30 column at a 24 mL/h flow rate, and their protein content was monitored by recording the absorbance at 280 nm. The chromatograms were divided in four regions. The regions 1, 2 and 3 were considered for the calculation of the percentage of proteins in each region. The region 4 was considered to represent phenol used as preservative