Fig. 1

Chromatographic profiles of non-RGD disintegrin from C. d. collilineatus venom using RP-FPLC system. a C. d. collilineatus venom (30 mg) was applied on a C18 column (250 × 10 mm, 5 μm particles, 300 Å), at a flow rate of 5 mL/min and (b) Fraction 2 (200 μg) on a C18 column (250 × 4.6 mm, 3.6 μm particles), at a flow rate of 0.5 mL/min. Elution in both chromatograms was carried out in a segmented concentration gradient from 6.3 to 100% of solution B (80% ACN in 0.1% TFA, represented by the blue dashed line) and absorbance was monitored at 214 nm. Inset panel – whole chromatographic profile without magnification