Fig. 2

Isolation profile of non-RGD disintegrin from C. d. collilineatus venom. a Tris-tricine-SDS-PAGE (16.5%), in non-reduced conditions and the gel was stained with 0.2% Coomassie Brilliant Blue G-250. MW1 – molecular mass (97.0–14.4 kDa); Cdc – C. d. collilineatus venom; 2 – Fraction 2 from RP-FPLC first step; 6–8 – fractions 6, 7 and 8, respectively, from RP-FPLC second step; MW2 – molecular mass (26.6–1.06 kDa). b Mass spectrum of Fraction 6 eluted from RP-FPLC second step obtained by MALDI-TOF (positive linear mode) using α-cyano-4-hydroxycinnamic acid (α-CHCA) matrix