Fig. 4From: Deep sequencing analysis of toad Rhinella schneideri skin glands and partial biochemical characterization of its cutaneous secretionCaseinolytic activity assay. Azocasein degradation was determined spectrophotometrically. Different volumes of CS were incubated in presence of EDTA or PMSF. Buffer was used as negative control and Trypsin as positive control. The degradation was measured in triplicate and normalized to relative activity compared to TrypsinBack to article page