Snake venom L-amino acid oxidases: an overview on their antitumor effects
© Costa et al.; licensee BioMed Central Ltd. 2014
Received: 13 February 2014
Accepted: 26 May 2014
Published: 2 June 2014
The L-amino acid oxidases (LAAOs) constitute a major component of snake venoms and have been widely studied due to their widespread presence and various effects, such as apoptosis induction, cytotoxicity, induction and/or inhibition of platelet aggregation, hemorrhage, hemolysis, edema, as well as antimicrobial, antiparasitic and anti-HIV activities. The isolated and characterized snake venom LAAOs have become important research targets due to their potential biotechnological applications in pursuit for new drugs of interest in the scientific and medical fields. The current study discusses the antitumor effects of snake venom LAAOs described in the literature to date, highlighting the mechanisms of apoptosis induction proposed for this class of proteins.
KeywordsSnake venoms L-amino acid oxidases Antitumor effects Apoptosis
The L-amino acid oxidases (LAAOs, EC 220.127.116.11) are flavoenzymes found in such diverse organisms as bacteria, fungi, algae, fish, snails as well as venoms of snakes from the families Viperidae, Crotalidae and Elapidae [1–6].
LAAOs from various sources have been isolated and characterized biochemically, enzymatically and biologically, with the snake venom L-amino acid oxidases (SV-LAAOs) being the most studied enzymes of this family of proteins .
In general, SV-LAAOs are homodimers with molecular masses ranging from 120 to 150 kDa in their native form and 50 to 70 kDa in their monomeric forms, and isoelectric point (pI) between 4.4 and 8.12 [2, 14]. Interestingly, acidic, neutral and basic forms of SV-LAAOs can coexist in the same snake venom and may present distinct pharmacological properties .
Until the 1990s, the studies of SV-LAAOs mainly focused on their physicochemical and enzymatic activities whereas more recent studies have shown that SV-LAAOs present numerous biological and pharmacological effects, such as induction of apoptosis, cytotoxicity, inhibition and induction of platelet aggregation, hemorrhage, hemolysis, edema, as well as microbicidal, antiparasitic and anti-HIV activities [2, 7, 12, 16–21].
Although several SV-LAAOs have been characterized with diverse biological functions, the mechanisms by which these enzymes exert their activities are not fully understood. It is believed that the biological effects of SV-LAAOs is, at least partially, due to the hydrogen peroxide generated during the enzymatic reaction, since the presence of catalase, an agent that degrades H2O2, can inhibit the action of these enzymes .
Nowadays, there is great interest in the clinical use of substances from plants and animals for the treatment of diseases, leading to a search for compounds with modulating actions on the carcinogen metabolism, induction of DNA repair systems and activation or suppression of the cell cycle and apoptosis . Apoptotic processes and cell damage are some of the action mechanisms proposed for many SV-LAAOs, suggesting that these enzymes could be used as models for the development of more effective chemotherapeutic and other antitumor agents [2, 13, 23, 24].
Therefore, this review aims to discuss the cytotoxic effects and the induction of apoptosis in tumor cells by SV-LAAOs. This analysis can serve as an important tool for future research studies on L-amino acid oxidases from snake venoms with antitumor activity.
Antitumor potential of SV-LAAOs
Numerous studies of snake venoms show that SV-LAAOs are capable of promoting cytotoxicity in different cell lines, such as S180 (murine sarcoma 180 tumor), SKBR-3 (breast adenocarcinoma), Jurkat (human acute T cell leukemia), EAT (Ehrlich ascites tumor), B16F10 (murine melanoma), PC12 (rat adrenal gland pheochromocytoma), as well as in non-tumor cells (lymphocytes and macrophages) . It is noteworthy that the damage in normal cells is usually negligible when compared to the damage caused in tumor cells [20, 25–27]. Although the cytotoxicity mechanisms of SV-LAAOs have not been fully clarified, it is known that lipids present in cell membranes can be damaged by reactive oxygen species (ROS) [28, 29]. Considering that membranes of tumor cells present higher concentrations of lipids than normal cells, it is speculated that the hydrogen peroxide produced by LAAOs exerts direct action on the membrane of tumor cells, with lower toxicity on normal cells .
Araki et al. reported for the first time the apoptosis in vascular endothelial cells caused by hemorrhagic venoms. Shortly afterwards, two other groups of researchers showed that LAAOs from hemorrhagic venoms were primarily responsible for the apoptotic effect on these endothelial cells [32, 33]. Since then, many studies have described the apoptotic effect of LAAOs in different cell lines, suggesting this enzyme class is directly linked to the cytotoxic action of venoms [11, 13, 14, 27, 33, 34].
The effects of SV-LAAOs can be studied by analyzing the cell cycle, which is a set of processes through which a cell passes during its division. This process is divided into two phases: interphase and mitosis, with the interphase being subdivided into G0, G1, S and G2 [35, 36]. During the cell cycle, certain stops (checkpoints) occur in order to verify the conditions of the genetic material at the time of cell division; these verifications involve multiple cellular repair proteins (CDK, CKI; CHK), which control the inhibition or the progression of the cycle by different pathways . The generated DNA damage in G1, S or G2 must be repaired as it is the last possible defense against damaged DNA, and if not repaired, the cell proceeds to mitosis and shall initiate the production of defective cells (tumor cells) or undergo cell death by apoptosis [35, 36].
The term apoptosis has been proposed by Kerr et al. in 1992 to describe the pathway of programmed cell death during cell development, which plays an important role in the development and maintenance of higher organisms. This process is triggered by DNA damage caused by physical, chemical and/or biological agents, and can be defined by various morphological and biochemical characteristics, such as the exposure of phosphatidylserine to the outer leaflet of the plasma membrane, nuclear condensation and the cleavage of chromatin in oligonucleosomal fragments [34, 39, 40].
Once unleashed, the phenomenon of apoptosis activates molecular events that culminate in the activation of caspases, which are responsible for cell dismantling and death. The process of apoptosis can occur by two major pathways: the intrinsic (mitochondrial) and extrinsic (death receptor). The intrinsic pathway can be triggered by the action of different intracellular stress signals, such as irradiation, chemotherapeutic agents, viruses, bacteria and absence of cell growth factors, which converge on the mitochondria to induce the translocation of cytochrome c and SMAC (second mitochondria-derived activator of caspases) from these organelles to the cytosol, resulting in the presence of APAF-1 and activation of caspase-9. The extrinsic pathway is initiated by the binding of death receptors (DR) – such as Fas/CD95, TNFRI, DR3, DR4, DR5 and DR6 – to their respective ligands. The existing DR are cell surface molecules that have a cysteine-rich extracellular domain and an intracellular domain denominated DD (death domain) [41, 42].
The binding of Fas associated with DD (FADD) allows the recruitment of pro-caspase 8 to form the DISC (death-inducing signaling complex). Pro-caspase 8 is self-cleaved and transformed into active caspase 8, and then released into the cytoplasm, where it may act directly on the activation of caspase 3 (executioner phase of apoptosis), or act in the cleavage of Bid molecules that will reach the mitochondria, inducing the release of cytochrome c and SMAC. The cleavage of Bid represents the connection between the extrinsic and intrinsic pathways of apoptosis [41, 43].
The mitochondrial pathway is regulated by members of the Bcl-2 family, which are cytoplasmic proteins capable of integrating signals of survival or cell death generated in the intra- and extracellular medium . This family is divided into two classes: anti-apoptotic proteins (Bcl-2, Bcl-xL, Bcl-w, A1 and Mcl-1), whose function is to protect cells from death, and pro-apoptotic proteins (Bax, Bak, Bad, Bid, Bmf etc.) that sensitize or lead cells to apoptosis . The executioner pathway of apoptosis is common to both initiating pathways and is characterized by the activation of effector caspases, namely caspase-3, −6 and −7, and the cell-dismantling characteristic of apoptosis [45–47]. The balance of the interactions between pro- and anti-apoptotic proteins may define the occurrence of cell death.
Numerous studies have reported that apoptotic processes induced by LAAOs are partially explained by the generation of hydrogen peroxide (H2O2), a reactive oxygen species (ROS) that accumulates on the surface of cell membranes. It is widely accepted that increasing ROS concentrations promotes mitochondrial derangements that cause cell death [2, 7, 11, 13, 23, 27, 32–34, 48, 49]. In this context, several studies with SV-LAAOs evaluated their cytotoxic effects in the presence of catalase (known for its ability to degrade H2O2 to H2O and O2), revealing that in fact the toxic action of SV-LAAOs is practically annulled by this agent [2, 7, 50].
To evaluate the cytotoxic activity of SV-LAAOs, most studies make use of the colorimetric method for cytotoxicity proposed by Mosmann . Ahn et al. showed that the LAAO isolated from Ophiophagus hannah (king cobra) venom is cytotoxic for stomach cancer cells (SNU-1). LAAOs from Agkistrodon acutus (ACTX-6) and Bungarus fasciatus (BF-LAAO) showed cytotoxic effects on A549 cells (lung adenocarcinoma), with ACTX-6 presenting an IC50 of 20 μg/mL [23, 49]. Alves et al. assessed the cytotoxic effects of an LAAO isolated from Bothrops atrox venom (named BatroxLAAO) on various tumor cell lines, such as HL-60 (IC50 50 μg/mL), PC12, B16F10 and JURKAT (IC50 of 25 μg/mL for the three cell lines). Also, in the presence of catalase (150 U/mL), BatroxLAAO did not induce significant cell death on any of the tumor cell lines tested .
One study revealed the toxin Bl-LAAO from Bothrops leucurus venom presented a cytotoxic effect on the tumor cell lines MKN-45 (stomach cancer), RKO (colorectal cancer) and LL-24 (human fibroblasts), whereas around 25% of this cytotoxicity was inhibited in the presence of catalase (100 μg) .
Bregge-Silva et al. evaluated the cytotoxic effect of an LAAO (denominated LmLAAO) isolated from Lachesis muta snake venom on AGS (gastric adenocarcinoma) and MCF-7 (breast tumor) cells, with IC50 of 22.7 μg/mL and 1.41 μg/mL, respectively. The catalase (0.1 mg/mL) completely abolished the cytotoxic effects of LmLAAO on MCF-7 tumor cells.
Several SV-LAAOs isolated from different snake venoms have been described as able to induce cell death in different cell lines [14, 20, 53, 54]. A study with the LAAO isolated from Agkistrodon halys snake venom demonstrated the apoptotic action of this protein on murine lymphoblastic leukemia cells (L1210) by quantitatively analyzing the DNA fragmentation after treatment of cells with the protein. Twenty-four hours after treatment, death by necrosis was observed, suggesting that higher amounts of H2O2 were released during the enzymatic reaction. When cells were treated concomitantly with catalase, cell viability was not fully restored, indicating that the apoptotic activity of LAAOs cannot be explained completely by the generation of hydrogen peroxide .
Torii et al. evaluated the apoptotic effects of Apoxin I, an LAAO from Crotalus atrox snake venom. Authors showed that Apoxin I at 10 μg/mL of this venom induced condensation and fragmentation of chromatin in human umbilical endothelial cells, HL-60, A2780 (human ovarian carcinoma) and NK-3 (rat endothelial cells). At a concentration of 2.5 μg/mL, Apoxin I induced oligonucleosomal DNA fragmentation in HL-60; however, at lower concentrations, the toxin did not induce apoptosis in this lineage. This study also showed that the induction of apoptosis was completely abolished when the LAAO was inactivated by changes in temperature (70°C) or in the presence of catalase. It was also found that in the presence of a membrane antioxidant (trolox), the Apoxin I was not able to induce apoptosis in the tested cell lines. These findings suggest that the apoptotic effect caused by Apoxin I is related to the catalytic activity of the enzyme, which is responsible for the production and release of H2O2 that may be related to the oxidation of the cell membrane .
ACL LAO, isolated from Agkistrodon contortrix laticinctus venom, was also capable of inducing apoptosis in HL-60 cells. Twenty-four hours after treatment with 25 μg/mL of the toxin, a typical pattern of DNA fragmentation in apoptotic cells was observed . Low concentrations of another protein of this class, the VB-LAAO from Vipera berus berus venom, induced apoptosis in K562 and HeLa tumor cell lines, whereas at higher concentrations, this enzyme also induced necrosis in K562 cells .
To examine the apoptotic and necrotic effects induced by SV-LAAOs, two flow cytometry methods have been employed: Annexin V FITC and HFS (hypotonic fluorescent solution, containing 50 μg/mL of propidium iodide in 0.1% sodium citrate plus 1.0% Triton X-100). Cells in early apoptosis are positive for annexin V and negative for propidium iodide (PI), which indicates phosphatidylserine externalization and membrane integrity. The assessment of DNA content detected by the HFS method considers the incorporation of PI in isolated nuclei compatible with the diploid content, whereas apoptotic nuclei appear in the hypodiploid region of the histogram due to the fragmentation of the nucleus or the greater condensation of chromatin .
The apoptotic and necrotic effects of BatroxLAAO were analyzed by flow cytometry. This toxin induced cell death processes in different tumor cell lines, such as JURKAT, B16F10, PC12 and HL-60. The B16F10 and PC12 cell lines presented death by apoptosis (AV+), while JURKAT cells displayed death by necrosis (27% necrotic cells) . In HL-60, 50 μg/mL BatroxLAAO showed apoptotic effect in 28.6% and necrotic effect in 14.2% of cells, maintaining a cell viability of approximately 57% . These data corroborate the study by Ande et al., which evaluated the effects of CR-LAAO from Calloselasma rhodostoma venom on the viability of JURKAT leukemia cells and the influence of catalase on apoptosis induction. CR-LAAO induced necrosis (PI+) in JURKAT cells in a dose-dependent manner. However, in the presence of catalase, the number of necrotic cells was drastically reduced, and a corresponding increase in the number of apoptotic cells (AV+) was observed, probably related to the catalase treatment.
Other studies have demonstrated the induction of apoptosis promoted by SV-LAAOs by the increased percentages of hypodiploid nuclei in tumor cell lines. Wei et al. showed that after 12 hours of treatment with BF-LAAO, the concentrations of 0.03, 0.1, 0.3, 1.0 and 3.0 μg/mL induced respective apoptosis proportions of 3.7, 6.6, 14.0, 32.4 and 41.2% in A549 cells. Burin et al. conducted tests to assess the effect of BpirLAAO (from Bothrops pirajai venom) on HL-60 and HL-60.Bcr-Abl tumor cell lines. Their results showed a dose-dependent increase in the percentage of hypodiploid nuclei 18 hours after treatment.
Furthermore, to assess whether SV-LAAOs induced apoptosis by the intrinsic (mitochondrial) or extrinsic (death receptor) pathway, some studies evaluated the detection of caspases 3, 8 and 9. Alves et al. reported the activation of caspases 3 and 9 24 hours after treatment of PC12, HL-60, JURKAT and B16F10 cell lines with BatroxLAAO. In relation to BpirLAAO, Burin et al. observed activation of caspases 3, 8 and 9 18 hours after treatment of HL-60 and HL-60.Bcr-Abl cell lines with BpirLAAO. These results suggest that SV-LAAOs may act in the activation of the intrinsic and extrinsic pathways of apoptosis.
Currently, molecular biology assays such as the combination of reverse transcription with quantitative real-time polymerase chain reaction (RT-qPCR) have contributed much to the study of the apoptotic potential of SV-LAAOs. The detection of the expression of pro- and anti-apoptotic genes assists in determining the apoptosis pathway (intrinsic or extrinsic) activated by these enzymes. The LAAO from Agkistrodon acutus venom (named ACTX-8) induced apoptosis in HeLa cells mediated by the mitochondrial pathway, which was detected by verifying the translocation of Bax and Bad from the cytosol to the mitochondria .
Few studies have been conducted to assess the effects of SV-LAAOs on the cell cycle progression. de Melo Alves-Paiva et al. evaluated the cycle modulation and the induction of apoptosis in HL-60 cells treated with BatroxLAAO, showing that this toxin induced a delay in the G0/G1 phase. The authors suggested that this delay may prevent the initiation of DNA synthesis and, consequently, the replication of tumor cells, which could represent another possible mechanism by which SV-LAAOs display their antitumor effects. Similar results were observed when LAAO was isolated from Agkistrodon acutus venom (ACTX-6), which promoted a 15% increase of A549 cells in the G0/G1 phase compared to the untreated group . K562 and U937 cells presented that same delay profile in G1 and decreased number of cells in the G2/M phase after treatment with drCT-I isolated from Daboia russelli russelli venom .
Summary of some SV-LAAOs and the tumor cell lines in which they were tested
Tumor cell lines
MTT, DNA fragmentation
Activation of caspases 3 and 9
Agkistrodon contortrix laticinctus
Agkistrodon halys pallas
Activation of caspases
HL-60 and EAT
MTT and DNA fragmentation
Activation of caspases 3, 8 and 9
Activation of caspases
Vipera berus berus
HeLa and K562
The mechanisms by which SV-LAAOs induce apoptosis are still not known, but studies suggest that the H2O2 produced during the enzymatic reaction, the activation of caspases and/or the interaction of LAAOs with membrane receptors may be involved in this cell death process.
Conducting new studies to elucidate the action mechanisms of SV-LAAOs are necessary to develop novel therapeutic strategies with more directed actions, which would result in more effective chemotherapeutic and antitumor agents.
The authors would like to thank the State of São Paulo Research Foundation (FAPESP – grants n. 2011/02645-3, 2011/23236-4 and 2012/11963-1), the National Council for Scientific and Technological Development (CNPq – grant n. 159632/2011-0) and the Support Nucleus for Research on Animal Toxins (NAP-TOXAN-USP – grant n. 12–125432.1.3) for funding our research. FAC and SVS hold a CNPq Scholarship in Research Productivity levels 2 and 1B, respectively.
- Vallon O, Bulté L, Kuras R, Olive J, Wollman FA: Extensive accumulation of an extracellular L-amino acid oxidase during gametogenesis of Chlamydomonas reinhardtii . Eur J Biochem 1993, 215(2):351–360. 10.1111/j.1432-1033.1993.tb18041.xView ArticlePubMedGoogle Scholar
- Du XY, Clemetson KJ: Snake venom L-amino acid oxidases. Toxicon 2002, 40(6):659–665. 10.1016/S0041-0101(02)00102-2View ArticlePubMedGoogle Scholar
- Kamio M, Ko KC, Zheng S, Wang B, Collins SL, Gadda G, Tai PC, Derby CD: The chemistry of escapin: identification and quantification of the components in the complex mixture generated by an L-amino acid oxidase in the defensive secretion of the sea snail Aplysia californica . Chemistry 2009, 15(7):1597–1603. 10.1002/chem.200801696View ArticlePubMedGoogle Scholar
- Chen WM, Sheu FS, Sheu SY: Novel L-amino acid oxidase with algicidal activity against toxic cyanobacterium Microcystis aeruginosa synthesized by a bacterium Aquimarina sp . Enzyme Microb Technol 2011, 49(4):372–379. 10.1016/j.enzmictec.2011.06.016View ArticlePubMedGoogle Scholar
- Wang F, Li R, Xie M, Li A: The serum of rabbitfish ( Siganus oramin ) has antimicrobial activity to some pathogenic organisms and a novel serum L-amino acid oxidase is isolated. Fish Shellfish Immunol 2011, 30(4–5):1095–1108.View ArticlePubMedGoogle Scholar
- Nuutinen JT, Marttinen E, Soliymani R, Hildén K, Timonen S: L-amino acid oxidase of the fungus Hebeloma cylindrosporum displays substrate preference towards glutamate. Microbiology 2012, 158(Pt 1):272–283.View ArticlePubMedGoogle Scholar
- Guo C, Liu S, Yao Y, Zhang Q, Sun MZ: Past decade study of snake venom L-amino acid oxidase. Toxicon 2012, 60(3):302–311. 10.1016/j.toxicon.2012.05.001View ArticlePubMedGoogle Scholar
- Curti B, Ronchi S, Pilone Simonetta M: D- and L-amino acid oxidases. In Chemistry and biochemistry of flavoenzymes, Volume 3. Edited by: Müller F. Boca Raton: CRC Press; 1992:69–94.Google Scholar
- Kommoju PR, Macheroux P, Ghisla S: Molecular cloning, expression and purification of L-amino acid oxidase from the Malayan pit viper Calloselasma rhodostoma . Protein Expr Purif 2007, 52(1):89–95. 10.1016/j.pep.2006.09.016View ArticlePubMedGoogle Scholar
- Li R, Zhu S, Wu J, Wang W, Lu Q, Clemetson KJ: L-amino acid oxidase from Naja atra venom activates and binds to human platelets. Acta Biochim Biophys Sin (Shangai) 2008, 40(1):19–26. 10.1111/j.1745-7270.2008.00372.xView ArticleGoogle Scholar
- Rodrigues RS, da Silva JF, Boldrini França J, Fonseca FP, Otaviano AR, Henrique Silva F, Hamaguchi A, Magro AJ, Braz AS, dos Santos JI, Homsi-Brandeburgo MI, Fontes MR, Fuly AL, Soares AM, Rodrigues VM: Structural and functional properties of Bp-LAAO, a new L-amino acid oxidase isolated from Bothrops pauloensis snake venom. Biochimie 2009, 91(4):490–501. 10.1016/j.biochi.2008.12.004View ArticlePubMedGoogle Scholar
- Sun MZ, Guo C, Tian Y, Chen D, Greenaway FT, Liu S: Biochemical, functional and structural characterization of Akbu-LAAO: a novel snake venom L-amino acid oxidase from Agkistrodon blomhoffii ussurensis . Biochimie 2010, 92(4):343–349. 10.1016/j.biochi.2010.01.013View ArticlePubMedGoogle Scholar
- de Melo Alves-Paiva R, de Freitas Figueiredo R, Antonucci GA, Paiva HH, de Lourdes Pires Bianchi M, Rodrigues KC, Lucarini R, Caetano RC, Linhari Rodrigues Pietro RC, Gomes Martins CH, de Albuquerque S, Sampaio SV: Cell cycle arrest evidence, parasiticidal and bactericidal properties induced by L-amino acid oxidase from Bothrops atrox snake venom. Biochimie 2011, 93(5):941–947. 10.1016/j.biochi.2011.01.009View ArticlePubMedGoogle Scholar
- Souza DH, Eugenio LM, Fletcher JE, Jiang MS, Garratt RC, Oliva G, Selistre-de-Araújo HS: Isolation and structural characterization of a cytotoxic L-amino acid oxidase from Agkistrodon contortrix laticinctus snake venom: preliminary crystallographic data. Arch Biochem Biophys 1999, 368(2):285–290. 10.1006/abbi.1999.1287View ArticlePubMedGoogle Scholar
- Stiles BG, Sexton FW, Weinstein SA: Antibacterial effects of different snake venoms: purification and characterization of antibacterial proteins from Pseudechis australis (Australian king brown or mulga snake) venom. Toxicon 1991, 29(9):1129–1141. 10.1016/0041-0101(91)90210-IView ArticlePubMedGoogle Scholar
- Samel M, Tõnismägi K, Rönnholm G, Vija H, Siigur J, Kalkkinen N, Siigur E: L-amino acid oxidase from Naja naja oxinana venom. Comp Biochem Physiol B Biochem Mol Biol 2008, 149(4):572–580. 10.1016/j.cbpb.2007.11.008View ArticlePubMedGoogle Scholar
- Zhong SR, Jin Y, Wu JB, Jia YH, Xu GL, Wang GC, Xiong YL, Lu QM: Purification and characterization of a new L-amino acid oxidase from Daboia russellii siamensis venom. Toxicon 2009, 54(6):763–771. 10.1016/j.toxicon.2009.06.004View ArticlePubMedGoogle Scholar
- Lee ML, Tan NH, Fung SY, Sekaran SD: Antibacterial action of a heat-stable form of L-amino acid oxidase isolated from king cobra ( Ophiophagus hannah ) venom. Comp Biochem Physiol C Toxicol Pharmacol 2011, 153(2):237–242. 10.1016/j.cbpc.2010.11.001View ArticlePubMedGoogle Scholar
- Naumann GB, Silva LF, Silva L, Faria G, Richardson M, Evangelista K, Kohlhoff M, Gontijo CM, Navdaev A, de Rezende FF, Eble JA, Sanchez EF: Cytotoxicity and inhibition of platelet aggregation caused by an L-amino acid oxidase from Bothrops leucurus venom. Biochim Biophys Acta 2011, 1810(7):683–694. 10.1016/j.bbagen.2011.04.003View ArticlePubMedGoogle Scholar
- Burin SM, Ayres LR, Neves RP, Ambrósio L, de Morais FR, Dias-Baruffi M, Sampaio SV, Pereira-Crott LS, de Castro FA: L-amino acid oxidase isolated from Bothrops pirajai induces apoptosis in BCR-ABL-positive cells and potentiates imatinib mesylate effect. Basic Clin Pharmacol Toxicol 2013, 113(2):103–112. 10.1111/bcpt.12073View ArticlePubMedGoogle Scholar
- Vargas LJ, Quintana JC, Pereañez JA, Núñez V, Sanz L, Calvete J: Cloning and characterization of an antibacterial L-amino acid oxidase from Crotalus durissus cumanensis venom. Toxicon 2013, 64: 1–11. doi:10.1016/j.toxicon.2012.11.027View ArticlePubMedGoogle Scholar
- Pieme CA, Guru SK, Ambassa P, Kumar S, Ngameni B, Ngogang JY, Bhushan S, Saxena AK: Induction of mitochondrial dependent apoptosis and cell cycle arrest in human promyelocytic leukemia HL-60 cells by an extract from Dorstenia psilurus : a spice from Cameroon. BMC Complement Altern Med 2013, 13: 223–231. doi:10.1186/1472–6882–13–223 10.1186/1472-6882-13-223PubMed CentralView ArticlePubMedGoogle Scholar
- Zhang L, Wu WT: Isolation and characterization of ACTX-6: a cytotoxic L-amino acid oxidase from Agkistrodon acutus snake venom. Nat Prod Res 2008, 22(6):554–563. 10.1080/14786410701592679View ArticlePubMedGoogle Scholar
- Marcussi S, Stábeli RG, Santos-Filho NA, Menaldo DL, Silva Pereira LL, Zuliani JP, Calderon LA, da Silva SL, Antunes LM, Soares AM: Genotoxic effect of Bothrops snake venoms and isolated toxins on human lymphocyte DNA. Toxicon 2013, 65: 9–14. doi: 10.1016/j.toxicon.2012.12.020View ArticlePubMedGoogle Scholar
- Ahn MY, Lee BM, Kim YS: Characterization and cytotoxicity of L-amino acid oxidase from the venom of King Cobra ( Ophiophagus hannah ). Int J Biochem Cell Biol 1997, 29(6):911–919. 10.1016/S1357-2725(97)00024-1View ArticlePubMedGoogle Scholar
- Izidoro LFM, Ribeiro MC, Souza GR, Sant’Ana CD, Hamaguchi A, Homsi-Brandeburgo MI, Goulart LR, Beleboni RO, Nomizo A, Sampaio SV, Soares AM, Rodrigues VM: Biochemical and functional characterization of an L-amino acid oxidase isolated from Bothrops pirajai snake venom. Bioorg Med Chem 2006, 14(20):7034–7043. 10.1016/j.bmc.2006.06.025View ArticlePubMedGoogle Scholar
- Alves RM, Antonucci GA, Paiva HH, Cintra ACO, Franco JJ, Mendonça-Franqueiro EP, Dorta DJ, Giglio JR, Rosa JC, Fuly AL, Dias-Baruffi M, Soares AM, Sampaio SV: Evidence of caspase-mediated apoptosis induced by L-amino acid oxidase isolated from Bothrops atrox snake venom. Comp Biochem Physiol A Mol Integr Physiol 2008, 151(4):542–550. 10.1016/j.cbpa.2008.07.007View ArticlePubMedGoogle Scholar
- Imlay JA: Pathways of oxidative damage. Annu Rev Microbiol 2003, 57: 395–418. 10.1146/annurev.micro.57.030502.090938View ArticlePubMedGoogle Scholar
- Okubo BM, Silva ON, Migliolo L, Gomes DG, Porto WF, Batista CL, Ramos CS, Holanda HHS, Dias SC, Franco OL, Moreno SE: Evaluation of an antimicrobial L-amino acid oxidase and peptide derivatives from Bothropoides mattogrosensis pitviper venom. PLoS ONE 2012, 7(3):e33639. 10.1371/journal.pone.0033639PubMed CentralView ArticlePubMedGoogle Scholar
- Yang CA, Cheng CH, Liu SY, Lo CT, Lee JW, Peng KC: Identification of antibacterial mechanism of L-amino acid oxidase derived from Trichoderma harzianum ETS 323. FEBS J 2011, 278(18):3381–3394. 10.1111/j.1742-4658.2011.08262.xView ArticlePubMedGoogle Scholar
- Araki S, Ishida T, Yamamoto T, Kaji K, Hayashi H: Induction of apoptosis by hemorrhagic snake venom in vascular endothelial cells. Biochem Biophys Res Commun 1993, 190(1):148–153. 10.1006/bbrc.1993.1023View ArticlePubMedGoogle Scholar
- Suhr SM, Kim DS: Identification of the snake venom substance that induces apoptosis. Biochem Biophys Res Commun 1996, 224(1):134–139. 10.1006/bbrc.1996.0996View ArticlePubMedGoogle Scholar
- Torii S, Naito M, Tsuruo T: Apoxin I, a novel apoptosis-inducing factor with L-amino acid oxidase activity purified from Western diamondback rattlesnake venom. J Biol Chem 1997, 272(14):9539–9542. 10.1074/jbc.272.14.9539View ArticlePubMedGoogle Scholar
- Ande SR, Kommoju PR, Draxl S, Murkovic M, Macheroux P, Ghisla S, Ferrando-May E: Mechanisms of cell death induction by L-amino acid oxidase, a major component of ophidian venom. Apoptosis 2006, 11(8):1439–1451. 10.1007/s10495-006-7959-9View ArticlePubMedGoogle Scholar
- Schafer KA: The cell cycle: a review. Vet Pathol 1998, 35(6):461–478. 10.1177/030098589803500601View ArticlePubMedGoogle Scholar
- Douglas RM, Haddad GG: Invited review: effect of oxygen deprivation on cell cycle activity: a profile of delay and arrest. J Appl Physiol 2003, 94(5):2068–2083.View ArticlePubMedGoogle Scholar
- Vermeulen K, Van Bockstaele DR, Berneman ZN: The cell cycle: a review of regulation, deregulation and therapeutic targets in cancer. Cell Prolif 2003, 36(3):131–149. 10.1046/j.1365-2184.2003.00266.xView ArticlePubMedGoogle Scholar
- Kerr JF, Winterford CM, Harmon BV: Apoptosis. Its significance in cancer and cancer therapy. Cancer 1994, 73(8):2013–2026. 10.1002/1097-0142(19940415)73:8<2013::AID-CNCR2820730802>3.0.CO;2-JView ArticlePubMedGoogle Scholar
- Dypbukt JM, Ankarcrona M, Burkitt M, Sjoholm A, Strom K, Orrenius S, Nicotera P: Different prooxidant levels stimulate growth, trigger apoptosis, or produce necrosis of insulin-secreting RINm5F cells. The role of intracellular polyamines. J Biol Chem 1994, 269(48):30553–30560.PubMedGoogle Scholar
- Bonfoco E, Krainc D, Ankarcrona M, Nicotera P, Lipton SA: Apoptosis and necrosis: two distinct events induced, respectively, by mild and intense insults with N-methyl-D-aspartate or nitric oxide/superoxide in cortical cell cultures. Proc Natl Acad Sci U S A 1995, 92(16):7162–7166. 10.1073/pnas.92.16.7162PubMed CentralView ArticlePubMedGoogle Scholar
- Amarante-Mendes GP, Green DR: The regulation of apoptotic cell death. Braz J Med Biol Res 1999, 32(9):1053–1061.View ArticlePubMedGoogle Scholar
- Pereira WO, Amarante-Mendes GP: Apoptosis: a programme of cell death or cell disposal? Scand J Immunol 2011, 73(5):401–407. 10.1111/j.1365-3083.2011.02513.xView ArticlePubMedGoogle Scholar
- Zivny J, Klener P Jr, Pytlik R, Andera L: The role of apoptosis in cancer development and treatment: focusing on the development and treatment of hematologic malignancies. Curr Pharm Des 2010, 16(1):11–33. 10.2174/138161210789941883View ArticlePubMedGoogle Scholar
- Borner C: The Bcl-2 protein family: sensors and checkpoints for life-or-death decisions. Mol Immunol 2003, 39(11):615–647. 10.1016/S0161-5890(02)00252-3View ArticlePubMedGoogle Scholar
- Du C, Fang M, Li Y, Li L, Wang X: Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition. Cell 2000, 102(1):33–42. 10.1016/S0092-8674(00)00008-8View ArticlePubMedGoogle Scholar
- Sharma K, Wang RX, Zhang LY, Yin DL, Luo XY, Solomon JC, Jiang RF, Markos K, Davidson W, Scott DW, Shi YF: Death the Fas way: regulation and pathophysiology of CD95 and its ligand. Pharmacol Ther 2000, 88(3):333–347. 10.1016/S0163-7258(00)00096-6View ArticlePubMedGoogle Scholar
- Susin SA, Lorenzo HK, Zamzami N, Marzo I, Snow BE, Brothers GM, Mangion J, Jacotot E, Costantini P, Loeffler M, Larochette N, Goodlett DR, Aebersold R, Siderovski DP, Penninger JM, Kroemer G: Molecular characterization of mitochondrial apoptosis-inducing factor. Nature 1999, 397(6718):441–446. 10.1038/17135View ArticlePubMedGoogle Scholar
- Zhang H, Teng M, Niu L, Wang Y, Wang Y, Liu Q, Huang Q, Hao Q, Dong Y, Liu P: Purification, partial characterization, crystallization and structural determination of AHP-LAAO, a novel L-amino acid oxidase with cell apoptosis-inducing activity from Agkistrodon halys pallas venom. Acta Crystallogr D Biol Crystallogr 2004, 60(Pt 5):974–977.View ArticlePubMedGoogle Scholar
- Wei JF, Yang HW, Wei XL, Qiao LY, Wang WY, He SH: Purification, characterization and biological activities of the L-amino acid oxidase from Bungarus fasciatus snake venom. Toxicon 2009, 54(3):262–271. 10.1016/j.toxicon.2009.04.017View ArticlePubMedGoogle Scholar
- Rojkind M, Domínguez-Rosales JA, Nieto N, Greenwel P: Role of hydrogen peroxide and oxidative stress in healing responses. Cell Mol Life Sci 2002, 59(11):1872–1891. 10.1007/PL00012511View ArticlePubMedGoogle Scholar
- Mosmann T: Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 1983, 65(1–2):55–63.View ArticlePubMedGoogle Scholar
- Bregge-Silva C, Nonato MC, de Albuquerque S, Ho PL, de Azevedo IL J, Vasconcelos Diniz MR, Lomonte B, Rucavado A, Díaz C, Gutiérrez JM, Arantes EC: Isolation and biochemical, functional and structural characterization of a novel L-amino acid oxidase from Lachesis muta snake venom. Toxicon 2012, 60(7):1263–1276. 10.1016/j.toxicon.2012.08.008View ArticlePubMedGoogle Scholar
- Ali SA, Stoeva S, Abbasi A, Alam JM, Kayed R, Faigle M, Neumeister B, Voelter W: Isolation, structural, and functional characterization of an apoptosis-inducing L-amino acid oxidase from leaf-nosed viper ( Eristocophis macmahoni ) snake venom. Arch Biochem Biophys 2000, 384(2):216–226. 10.1006/abbi.2000.2130View ArticlePubMedGoogle Scholar
- Torii S, Yamane K, Mashima T, Haga N, Yamamoto K, Fox JW, Naito M, Tsuruo T: Molecular cloning and functional analysis of apoxin I, a snake venom-derived apoptosis-inducing factor with L-amino acid oxidase activity. Biochemistry 2000, 39(12):3197–3205. 10.1021/bi992416zView ArticlePubMedGoogle Scholar
- Samel M, Vija H, Ronnholm G, Siigur J, Kalkkinen N, Siigur E: Isolation and characterization of an apoptotic and platelet aggregation inhibiting L-amino acid oxidase from Vipera berus berus (common viper) venom. Biochim Biophys Acta 2006, 1764(4):707–714. 10.1016/j.bbapap.2006.01.021View ArticlePubMedGoogle Scholar
- Nicoletti L, Migliorati G, Pagliacci MC, Grignani F, Riccardi C: A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J Immunol Methods 1991, 139(2):271–279. 10.1016/0022-1759(91)90198-OView ArticlePubMedGoogle Scholar
- Zhang L, Wei LJ: ACTX-8, a cytotoxic L-amino acid oxidase isolated from Agkistrodon acutus snake venom, induces apoptosis in Hela cervical cancer cells. Life Sci 2007, 80(13):1189–1197. 10.1016/j.lfs.2006.12.024View ArticlePubMedGoogle Scholar
- Gomes A, Choudhury SR, Saha A, Mishra R, Giri B, Biswas AK, Debnath A, Gomes A: A heat stable protein toxin (drCT-I) from the Indian Viper ( Daboia russelli russelli ) venom having antiproliferative, cytotoxic and apoptotic activities. Toxicon 2007, 49(1):46–56. 10.1016/j.toxicon.2006.09.009View ArticlePubMedGoogle Scholar
- Liu JW, Chai MQ, Du XY, Song JG, Zhou YC: Purification and characterization of L-amino acid oxidase from Agkistrodon halys pallas venom. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 2002, 34(3):305–310. Article in ChineseGoogle Scholar
- Stábeli RG, Sant’Ana CD, Ribeiro PH, Costa TR, Ticli FK, Pires MG, Nomizo A, Albuquerque S, Malta-Neto NR, Marins M, Sampaio SV, Soares AM: Cytotoxic L-amino acid oxidase from Bothrops moojeni : biochemical and functional characterization. Int J Biol Macromol 2007, 41(2):132–140. 10.1016/j.ijbiomac.2007.01.006View ArticlePubMedGoogle Scholar
- Lee ML, Chung I, Fung SY, Kanthimathi MS, Tan NH: Anti-proliferative activity of king cobra ( Ophiophagus hannah ) venom L-amino acid oxidase. Basic Clin Pharmacol Toxicol 2013. doi:10.1111/bcpt.12155Google Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.